Virtual Workshop
October 19, 2021
Objectives
The goal of the Plant Cell Atlas (PCA) is to bring together a community who will comprehensively describe plant cell types by integrating high-resolution subcellular and cellular location information of nucleic acids, proteins, and metabolites. In line with achieving this goal, metabolomics provides a functional readout of the cellular and molecular programs controlled through expression of genes and proteins, as metabolites are directly linked to cellular function, response to environmental stresses, and progression to disease. Significant advances in mass spectrometry imaging and spatially resolved mass spectrometry technologies now make high-spatial resolution metabolomics notably more accessible for plant science. This PCA workshop aims to bring together world leaders in spatial and mass spectrometry imaging-based metabolomics from plant sciences and beyond to help realize the development of a comprehensive PCA metabolome.
Workshop Times
PDT – 8:00am – 11:00am
MDT – 9:00am – 12:00pm
CDT – 10:00am – 1:00pm
EDT – 11:00am – 2:00pm
CEST – 5:00pm – 8:00pm
GMT+8 – 11:00pm – 2:00am (10/20/2021)
Recordings of the three of the talks can be found on the PCA YouTube channel.
Agenda
| 8:00 AM – 8:05 AM PDT | Introduction |
| 8:05 AM – 8:30 AM PDT | Akos Vertes (George Washington University) Talk Title: Basic concepts in single cell analysis and spatial metabolomics of plant tissues |
| 8:30 AM – 8:55 AM PDT | Theodore Alexandrov (European Molecular Biology Laboratory – EMBL) Talk Title: METASPACE and SpaceM, tools for spatial single-cell metabolomics |
| 8:55 AM – 9:20 AM PDT | Hua Tian (Pennsylvania State University) Talk Title: Successive high-resolution (H2O)n-GCIB and C60-SIMS imaging integrates spatial omics (metabolites, lipids and proteins) in single cells on frozen-hydrated tissues |
| 9:20 AM – 9:35 AM PDT | Break |
| 9:35 AM – 10:00 AM PDT | Ingela Lanekoff (Uppsala University) Talk Title: Quantitative spatial mapping and in-depth characterization of metabolites and lipids |
| 10:00 AM – 10:25 AM PDT | Young-Jin Lee (Iowa State University) Talk Title: High-spatial resolution mass spectrometry imaging of plant metabolites with matrix-assisted laser desorption ionization |
| 10:25 AM – 11:00 AM PDT | Panel Discussion: Future outlook for single cell metabolomics in plants |
Workshop Organizers and Speakers
Organizing Committee
Spatial Metabolomics Workshop
Christopher Anderton, PhD
Pacific Northwest National Laboratory
Toshihiro Obata, PhD
University of Nebraska, Lincoln
Gary Stacey, PhD
University of Missouri
Speakers
Theodore Alexandrov, PhD
European Molecular Biology Laboratory
Ingela Lanekoff, PhD
Uppsala University
Young-Jin Lee, PhD
Iowa State University
Hua Tian, PhD
Pennsylvania State University
