Virtual Workshop
May 19, 2022
The goal of the Plant Cell Atlas (PCA) is to bring together a community who will comprehensively describe plant cell types by integrating high-resolution subcellular and cellular location information of nucleic acids, proteins, and metabolites. In particular, spatial-omics techniques are rapidly advancing and transforming the way we understand complex organisms and their interactions with the environment. One form, termed “multiplex” microscopy, has been increasingly applied in complex tissue systems to understand disease and development. Multiplex microscopy simultaneously and/or sequentially visualizes an extended array of biomolecular probes on single sections/cell monolayers and intact tissues and organisms, thus providing high-dimensional physical mapping of numerous cellular phenotypes and components over heterogeneous tissues and complements other high-resolution omics such as single-cell sequencing. This PCA symposium brings together pioneers, early adopters and commercial experts in a broad array of cutting-edge multiplex imaging and analysis approaches to encourage further development and adoption by the plant science community.
Registration is closed for the PCA Multiplex Microscopy Symposium.
Workshop Times
PDT – 10:30am – 3:05pm
MDT – 11:30am – 4:05pm
CDT – 12:30pm – 5:05pm
EDT – 1:30pm – 6:05pm
CEST – 6:30pm – 8:05pm
GMT+8 – 12:30am – 6:05am (5/20/2022)
Search for your time zone here.
Recordings of the two of the talks can be found on the PCA YouTube channel.
Organizers
Kirk Czymmek, PhD – Donald Danforth Center
Kun Huang, PhD – Dana-Farber Cancer Institute
Speakers
- Regan Baird (Visiopharm)
Talk Topic: Multiplex Image Processing/Analysis - Jiang He (Vizgen)
Talk Topic: Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH) Method - Sizun Jiang (Beth Israel Deaconess Medical Center)
Talk Topic: CODEX, Multiplexed Ion Beam Imaging (MIBI) - Yangning Lu (Massachusetts Institute of Technology)
Talk Topic: Multiplex Expansion Microscopy - Lingyan Shi (University of California, San Diego)
Talk Topic: Stimulated Raman Scattering (SRS) – Multiplex Metabolism - Peng Yin (Harvard University)
Talk Topic: DNA-based imaging
Agenda
| 10:30 AM – 10:35 AM PDT | Opening Remarks |
| 10:35 AM – 11:15 AM PDT | Lingyan Shi (University of California, San Diego)Talk Topic: Stimulated Raman Scattering (SRS) – Multiplex Metabolism |
| 11:15 AM – 11:55 AM PDT | Peng Yin (Harvard University) Talk Topic: DNA probes for multiplexed imaging: DNA-Exchange, DNA-PAINT, and SABER |
| 11:55 AM – 12:35 PM PDT | Jiang He (Vizgen)Talk Topic: Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH) Method |
| 12:35 PM – 12:50 PM PDT | Break |
| 12:50 PM – 1:30 PM PDT | Yangning Lu (Massachusetts Institute of Technology) Talk Topic: Multiplex Expansion Microscopy |
| 1:30 PM – 2:10 PM PDT | Regan Baird (Visiopharm) Talk Topic: Multiplex Image Processing/Analysis |
| 2:10 PM – 3:00 PM PDT | Sizun Jiang (Beth Israel Deaconess Medical Center) Talk Topic: CODEX, Multiplexed Ion Beam Imaging (MIBI) |
| 3:00 PM – 3:05 PM PDT | Closing Remarks |